This is a small device which used to septate protein, DNA or RNA. Nucleic acid are size separated by the aid of an electric field. negatively charged molecule migrate toward anode and this solely determined by the molecule weight. Where small weight molecule migrate faster than large ones.
Voltage
Migration of fragment depends on the electric current. Different optimal voltages are required for different fragment sizes. As a example best fragmentation for molecule larger than 2Kb could be obtained by applying no more than 5 volts per centimetre.
Safety
PM check-list
1. Voltage should be smoothly adjustable.2. Electrode must be clean to ensure proper connection in between power supply and gel.
3. Voltage different can be checked in between one centimetre whether it correct or not.
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